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Ascorbate Oxidase Antikörper (HRP)

AO Reaktivität: Pumpkin ELISA, WB, IP Wirt: Kaninchen Polyclonal HRP
Produktnummer ABIN5596757
  • Target Alle Ascorbate Oxidase (AO) Produkte
    Ascorbate Oxidase (AO)
    Reaktivität
    • 7
    • 2
    Pumpkin
    Wirt
    • 9
    Kaninchen
    Klonalität
    • 9
    Polyklonal
    Konjugat
    • 5
    • 4
    Dieser Ascorbate Oxidase Antikörper ist konjugiert mit HRP
    Applikation
    • 8
    • 8
    • 3
    • 2
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunoprecipitation (IP)
    Kreuzreaktivität (Details)
    Cross reactivity against Ascorbate Oxidase from other sources is unknown.
    Aufreinigung
    Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].
    Immunogen

    Immunogen: Ascorbate Oxidase [cucurbita]

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise

    Application Note: Anti-Ascorbate Oxidase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:3,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Konservierungsmittel
    Gentamicin sulfate
    Vorsichtsmaßnahmen
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    Ascorbate Oxidase (AO)
    Andere Bezeichnung
    Ascorbate Oxidase (AO Produkte)
    Synonyme
    AO antikoerper, ascorbate oxidase antikoerper, L-ascorbate oxidase antikoerper, AO antikoerper, LOC9310099 antikoerper
    Hintergrund

    Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3

    Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.

    Gene Name: AAO

    UniProt
    P24792
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