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Fibronectin 1 Antikörper (Biotin)

Rockland 600-406-117 FN1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Affe WB, ELISA, IHC, IP Wirt: Kaninchen Polyclonal Biotin
Rockland
Produktnummer ABIN5596762
Hersteller Produkt- Nr.: 600-406-117
  • Target Alle Fibronectin 1 (FN1) Antikörper anzeigen
    Fibronectin 1 (FN1)
    Reaktivität
    • 91
    • 35
    • 30
    • 8
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Rind (Kuh), Affe
    Wirt
    • 73
    • 31
    • 1
    • 1
    Kaninchen
    Klonalität
    • 72
    • 33
    Polyklonal
    Konjugat
    • 59
    • 14
    • 9
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Fibronectin 1 Antikörper ist konjugiert mit Biotin
    Applikation
    • 67
    • 45
    • 23
    • 14
    • 13
    • 13
    • 11
    • 9
    • 9
    • 8
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Hersteller Produkt- Nr.
    600-406-117
    Hersteller
    Rockland
    Verwendungszweck
    Fibronectin Antibody Biotin Conjugated
    Kreuzreaktivität (Details)
    Typically less than 1 % cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
    Produktmerkmale
    Synonyms: rabbit anti-Fibronectin antibody biotin conjugation, biotin conjugated rabbit anti-Fibronectin antibody, FN1, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3
    Aufreinigung
    Anti-fibronectin (rabbit) antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificity.
    Immunogen

    Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.

    Immunogen Type: Native Protein

    Isotyp
    IgG
    Labeling Ratio
    10-20
    Top Product
    Discover our top product FN1 Primärantikörper
    Produktspezifische Information

    Dieser polyklonale Kaninchen anti-Fibronektin Antikörper ist Biotin-konjugiert und eignet sich zur Detektion von Fibronektin (FN, FN1) in den Spezies Mensch, Maus, Ratte, Rind, Affe. Der Fibronektin Antikörper ist validiert für ELISA, Immunohistochemie, Immunopräzipitation und Westernblotting. Als Beleg für die Performance des Antikörpers liegen derzeit vier Produktbilder für IHC und das unfolding profile vor. Nutzen Sie unseren hochwertigen Fibronectin Antikörper, um Fibronektin zuverlässig für eine Reihe von Spezies nachzuweisen.

    Fibronektine binden Zelloberflächen und verschiedene Verbindungen wie Kollagen, Fibrin, Heparin, DNA und Aktin. Fibronektine sind an der Zelladhäsion, Zellmotilität, Opsonisierung, Wundheilung und Aufrechterhaltung der Zellform beteiligt. Ist an der Osteoblastenverdichtung durch den zellvermittelten Prozess der Fibronektin-Fibrillogenese beteiligt, der für die Mineralisierung der Osteoblasten unerlässlich ist (By similarity). Beteiligt sich an der Regulierung der Ablagerung von Typ-I-Kollagen durch Osteoblasten (durch Ähnlichkeit). (UniProt)

  • Applikationshinweise

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-Fibronectin (rabbit) antibody was tested by WB, ELISA, and IHC. Assay by immunoblot was found to be reactive against Fibronectin at a dilution of 1:5,000 to 1:10,000.  Assay against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.   A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product.  For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #104084 (Unfolding Profile)
    'Independent Validation' Siegel
    by
    NanoTemper Technologies
    No.
    #104084
    Datum
    23.07.2019
    Antigen
    Fibronectin
    Chargennummer
    39102
    Validierte Anwendung
    Unfolding Profile
    Positivkontrolle
    ABIN5596762
    Negativkontrolle
    Bewertung

    Passed. ABIN5596762 showed Ti at 63.4°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    Sekundärantikörper
    Full Protocol
    • Dilute ABIN5596762 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 0.5µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Anmerkungen

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    Fibronectin 1 (FN1)
    Andere Bezeichnung
    FN1 (FN1 Produkte)
    Hintergrund
    Background: Fibronectin antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin, an attraction that is the strongest and most stable non-covalent interaction known. Fibronectin is found in two forms in vertebrates: soluble and insoluble. Soluble plasma fibronectin is contained in blood plasma and constitutes a large protein component. Insoluble cellular fibronectin is a large component of the extra-cellular matrix where it is secreted by many different types of cells. Fibronectin plays a large role in wound healing and cell development. Anti-fibronectin (rabbit) antibody is ideal for investigators in Cardiology, Cell Biology, Microbiology, and Immunology research.
    Gen-ID
    2335
    UniProt
    P02751
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie
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