Pyruvate Kinase Antikörper (HRP)

Produktnummer ABIN5596779

Produktdetails anti-Pyruvate Kinase Antikörper (HRP)

Target: Pyruvate Kinase (PK)

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Pyruvate Kinase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Pyruvate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Immunogen:

Immunogen: Pyruvate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Pyruvate Kinase has been assayed against 1.0 μg of Pyruvate Kinase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Andere Bezeichnung: Pyruvate Kinase (PK Produkte)

Synonyme: CG7070 antikoerper, Dmel\\CG7070 antikoerper, PYK antikoerper, Pyk antikoerper, PYK3b antikoerper, PYK3c antikoerper, Pyruvate kinase antikoerper, PK antikoerper, pyruvate kinase antikoerper, pyruvate kinase (PK) antikoerper, Pyruvate kinase (PK) antikoerper, pyruvate kinase PKM antikoerper, testis expressed 45 antikoerper, PyK antikoerper, pykA antikoerper, PYK3a|PYK3b|PYK3c antikoerper, pyk antikoerper, pyk1 antikoerper, pk antikoerper, LOC100534543 antikoerper, TEX45 antikoerper

Hintergrund:

Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, HRP

Background: Pyruvate Kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Genetic defects of this enzyme cause the disease known as pyruvate kinase deficiency and can cause hemolytic anemia.

Gene Name: PKM2

Gen-ID: 100008676

UniProt: P11974