Acetylcholinesterase Antikörper (HRP)

Produktnummer ABIN5596812

Produktdetails anti-Acetylcholinesterase Antikörper (HRP)

Target: Acetylcholinesterase (AChE)

Reaktivität: Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Acetylcholinesterase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Acetylcholinesterase from other sources is unknown.

Aufreinigung: Anti-Acetylcholinesterase (Bovine Erthryocytes) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Acetylcholinesterase [Bovine Erythrocytes].

Immunogen:

Immunogen: Acetylcholinesterase [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Acetylcholinesterase (Bovine Erthryocytes) antibody has been assayed against 1.0 μg of Acetylcholinesterase [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: Acetylcholinesterase (AChE Produkte)

Synonyme: ACEE antikoerper, ARACHE antikoerper, N-ACHE antikoerper, YT antikoerper, Ache antikoerper, GB14873 antikoerper, zgc:92550 antikoerper, ACE antikoerper, Dsim\\GD20515 antikoerper, GD20515 antikoerper, dsim_GLEANR_4292 antikoerper, ache antikoerper, mE1a antikoerper, mE1b antikoerper, mE1c antikoerper, mE1c-long antikoerper, mE1d antikoerper, mE1d' antikoerper, mE1e antikoerper, arache antikoerper, n-ache antikoerper, acetylcholinesterase (Cartwright blood group) antikoerper, acetylcholinesterase 2 antikoerper, acetylcholinesterase antikoerper, Acetylcholine esterase antikoerper, acetylcholinesterase (Cartwright blood group) L homeolog antikoerper, collagen type I alpha 2 chain antikoerper, ACHE antikoerper, AChE-2 antikoerper, Ache antikoerper, ache antikoerper, Dsim\Ace antikoerper, ache.L antikoerper, COL1A2 antikoerper, ACE-1 antikoerper

Hintergrund:

Synonyms: Acetylcholinesterase, AChE, HRP, Horseradish peroxidase

Background: Acetylcholinesterase, or AChE, is an important protease that terminates the transmission of the neurotransmitter, acetylcholine, by hydrolysis. Hydrolysis by AChE on ACh prevents excessive neural firing between adjacent nerves. Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Acetylcholinesterase (Bovine Erthryocytes) antibody is ideal for investigators in Signal Transduction, Cell Signaling, Cardiovascular , and Immunology research.

Gene Name: ACHE

Gen-ID: 540446

UniProt: P23795

Pathways: Skeletal Muscle Fiber Development