Acid Phosphatase Antikörper (HRP)

Produktnummer ABIN5596813

Produktdetails anti-Acid Phosphatase Antikörper (HRP)

Target: Acid Phosphatase (ACP)

Reaktivität: Potato

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Acid Phosphatase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Acid Phosphatase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].

Immunogen:

Immunogen: Acid Phosphatase [Potato]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Acid Phosphatase is suitable for use in ELISA, western blot, and immunohistochemistry. Optimal titers should be optimized by researcher.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Acid Phosphatase

Target: Acid Phosphatase (ACP)

Andere Bezeichnung: Acid Phosphatase (ACP Produkte)

Synonyme: Acph antikoerper, ACP antikoerper, Acid phosphatase antikoerper, acid phosphatase antikoerper, cerebral palsy, ataxic 1 antikoerper, Dana\Acph antikoerper, phoC antikoerper, CPAT1 antikoerper

Hintergrund:

Synonyms: Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

Background: Acid Phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function, therefore, it has an acid pH optimum. Different forms of acid phosphatase are found in different organs, and their serum levels are used to evaluate the success of the surgical treatment of prostate cancer.

Gene Name: PAP3

UniProt: Q6J5M8