Aspartate Transaminase Antikörper (HRP)

Produktnummer ABIN5596816

Hersteller Produkt- Nr.: 200-603-144-0100

Dieses Anti-Aspartate Transaminase-Antikörper ist ein Schaf Polyklonal-Antikörper zur Detektion von Aspartate Transaminase in ELISA, WB und DB. Geeignet für Schwein.

Kurzübersicht für Aspartate Transaminase Antikörper (HRP) (ABIN5596816)

Target: Aspartate Transaminase (Ast)

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Aspartate Transaminase Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-Aspartate Transaminase Antikörper (HRP)

Hersteller Produkt- Nr.: 200-603-144-0100

Hersteller: Rockland

Verwendungszweck: Aspartate Transaminase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].

Produktmerkmale: Synonyms: sheep anti-Aspartate Transaminase HRP conjugated Antibody, peroxidase conjugated sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

Aufreinigung: Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Aspartate Transaminase Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Aspartate Transaminase

Target: Aspartate Transaminase (Ast)

Andere Bezeichnung: Aspartate Transaminase

Hintergrund: Background: Aspartate Transaminase is an important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Aspartate Transaminase is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells, and it is commonly measured clinically as a marker for liver health.

Gen-ID: 396967

NCBI Accession: NP_999092

UniProt: P00503