COL2A1 Antikörper

Produktnummer ABIN5596824

Produktdetails anti-COL2A1 Antikörper

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reaktivität: Human, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL2A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM), Dot Blot (DB)

Verwendungszweck: Collagen Type II Antibody

Kreuzreaktivität (Details): Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Produktmerkmale: Synonyms: rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

Aufreinigung: Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein

Isotyp: IgG

Produktspezifische Information:

Für welche Zwecke kann der COL2-Antikörper ABIN5596824 verwendet werden? Dieser polyklonale, unkonjugierte Kaninchen-COL2-Antikörper eignet sich für den Nachweis von Kollagen Typ 2 (COL2) mittels ELISA, Immunhistochemie, Immunpräzipitation und Western Blotting. Der Antikörper erkennt COL2 in Mensch, Maus, Ratte, Rind, Schaf und anderen Säugetieren.

Welche Validierungsdaten liegen für diesen COL2-Antikörper vor? Das Produkt wurde von NanoTemper Technologies durch Bestimmung des Entfaltungsprofils unabhängig validiert. Es sind derzeit 2 Produktbilder verfügbar, die die Leistungsfähigkeit des COL2-Antikörpers zeigen. Nutzen Sie unseren Antikörper, um COL2 zuverlässig nachzuweisen.

Was ist die Funktion von COL2? Kollagen Typ 2 (COL2) ist spezifisch für Knorpelgewebe. Es ist wichtig für die normale embryonale Entwicklung des Skeletts, für das lineare Wachstum und für die Fähigkeit des Knorpels, Druckkräften zu widerstehen.

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:50 - 1:400

Application Note: Anti-Collagen Type II has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (UP)

Validierung #104083 (Unfolding Profile)

by: NanoTemper Technologies

No.: #104083

Datum: 23.07.2019

Antigen: COL2

Chargennummer: 41639

Validierte Anwendung: Unfolding Profile

Positivkontrolle: ABIN5596824

Bewertung:

Passed. ABIN5596824 showed T_i at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validierungsbilder

Unfolding profile of ABIN5596824. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 78.9°C.

Protokoll

Full Protocol:

• Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Anmerkungen:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Details zu COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Andere Bezeichnung: COL2A1 (COL2A1 Produkte)

Hintergrund: Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gen-ID: 1280

NCBI Accession: NP_001835

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding