COL2 Antikörper

Produktnummer ABIN5596824

Produktdetails anti-COL2 Antikörper

Target: COL2 (Collagen, Type II (COL2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Säugetier, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Kreuzreaktivität (Details): Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Aufreinigung: Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogen:

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein

Isotyp: IgG

Produktspezifische Information:

Für welche Zwecke kann der COL2-Antikörper ABIN5596824 verwendet werden? Dieser polyklonale, unkonjugierte Kaninchen-COL2-Antikörper eignet sich für den Nachweis von Kollagen Typ 2 (COL2) mittels ELISA, Immunhistochemie, Immunpräzipitation und Western Blotting. Der Antikörper erkennt COL2 in Mensch, Maus, Ratte, Rind, Schaf und anderen Säugetieren.

Welche Validierungsdaten liegen für diesen COL2-Antikörper vor? Das Produkt wurde von NanoTemper Technologies durch Bestimmung des Entfaltungsprofils unabhängig validiert. Es sind derzeit 2 Produktbilder verfügbar, die die Leistungsfähigkeit des COL2-Antikörpers zeigen. Nutzen Sie unseren Antikörper, um COL2 zuverlässig nachzuweisen.

Was ist die Funktion von COL2? Kollagen Typ 2 (COL2) ist spezifisch für Knorpelgewebe. Es ist wichtig für die normale embryonale Entwicklung des Skeletts, für das lineare Wachstum und für die Fähigkeit des Knorpels, Druckkräften zu widerstehen.

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:50 - 1:400

Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (UP)

Validierung #104083 (Unfolding Profile)

by: NanoTemper Technologies

No.: #104083

Datum: 23.07.2019

Antigen: COL2

Chargennummer: 41639

Validierte Anwendung: Unfolding Profile

Positivkontrolle: ABIN5596824

Bewertung:

Passed. ABIN5596824 showed T_i at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validierungsbilder

Unfolding profile of ABIN5596824. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 78.9°C.

Protokoll

Full Protocol:

• Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Anmerkungen:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu COL2

Target: COL2 (Collagen, Type II (COL2))

Andere Bezeichnung: Collagen Type II (COL2 Produkte)

Synonyme: PT antikoerper, RPRGL2 antikoerper, THPH1 antikoerper, Cf-2 antikoerper, Cf2 antikoerper, FII antikoerper, thrombin antikoerper, prothrombin antikoerper, CAP18 antikoerper, Cnlp antikoerper, Cramp antikoerper, FALL39 antikoerper, MCLP antikoerper, coagulation factor II, thrombin antikoerper, coagulation factor II antikoerper, cathelicidin antimicrobial peptide antikoerper, Cuticle collagen 2 antikoerper, collagen type II alpha 1 chain antikoerper, F2 antikoerper, Camp antikoerper, col-2 antikoerper, COL2A1 antikoerper

Hintergrund:

Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. We extensively purify collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gene Name: COL2A1

Gen-ID: 1280

UniProt: P02458