COL2A1 Antikörper

Produktnummer ABIN5596825

Hersteller Produkt- Nr.: 600-401-104-0.5

Produktdetails anti-COL2A1 Antikörper

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reaktivität: Human, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL2A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 600-401-104-0.5

Hersteller: Rockland

Verwendungszweck: Collagen Type II Antibody

Kreuzreaktivität (Details): Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Produktmerkmale: Synonyms: rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

Aufreinigung: Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:50 - 1:400

Application Note: Anti-Collagen Type II has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Referenzen für anti-COL2A1 Antikörper (ABIN5596825)

Kondo, Shukunami, Morioka, Matsumoto, Takahashi, Oh, Atsumi, Umezawa, Kudo, Kitayama, Hiraki, Noda: "Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells." in: Journal of cell science, Vol. 120, Issue Pt 5, pp. 849-57, (2007) (PubMed).

Details zu COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Andere Bezeichnung: COL2A1 (COL2A1 Produkte)

Hintergrund: Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gen-ID: 1280

NCBI Accession: NP_001835

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding