Collagen IV Antikörper (HRP)
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- Target Alle Collagen IV (COL4) Antikörper anzeigen
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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Reaktivität
- Human, Rind (Kuh), Säugetier
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Collagen IV Antikörper ist konjugiert mit HRP
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Kreuzreaktivität (Details)
- Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Aufreinigung
- Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- Immunogen
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Immunogen: Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.
Immunogen Type: Native Protein
- Isotyp
- IgG
- Top Product
- Discover our top product COL4 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Collagen Type IV Peroxidase Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:2,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:20,000 - 1:100,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL to 20 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Konservierungsmittel
- Thimerosal (Merthiolate)
- Vorsichtsmaßnahmen
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
- Haltbarkeit
- 12 months
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- Target
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- Andere Bezeichnung
- Collagen Type IV (COL4 Produkte)
- Synonyme
- collagen, type IV antikoerper, Let-2 antikoerper
- Hintergrund
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Synonyms: Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody
Background: Anti-Collagen Type IV detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Gene Name: COL4A4
- Gen-ID
- 1282
- UniProt
- P02462
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