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COL6 Antikörper

COL6 Reaktivität: Human, Rind (Kuh), Säugetier WB, ELISA, IHC, IP Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5596844
  • Target Alle COL6 Antikörper anzeigen
    COL6 (Collagen, Type VI (COL6))
    Reaktivität
    • 31
    • 10
    • 10
    • 5
    • 4
    • 1
    • 1
    • 1
    Human, Rind (Kuh), Säugetier
    Wirt
    • 21
    • 9
    • 1
    Kaninchen
    Klonalität
    • 22
    • 9
    Polyklonal
    Konjugat
    • 17
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser COL6 Antikörper ist unkonjugiert
    Applikation
    • 20
    • 18
    • 15
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Kreuzreaktivität (Details)
    Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Aufreinigung
    Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    Immunogen

    Immunogen: Collagen Type VI from human and bovine placenta

    Immunogen Type: Native Protein

    Isotyp
    IgG
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    Discover our top product COL6 Primärantikörper
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

    Western Blot Dilution: 1:1,000 - 1:10,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:50,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Ida, Hikage, Itoh, Ida, Ohguro: "Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix." in: Scientific reports, Vol. 10, Issue 1, pp. 7958, (2020) (PubMed).

  • Target
    COL6 (Collagen, Type VI (COL6))
    Andere Bezeichnung
    Collagen Type VI (COL6 Produkte)
    Hintergrund

    Synonyms: CollagenVI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody

    Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

    Gene Name: COL6A1

    Gen-ID
    1291
    UniProt
    P12109
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