Phosphothreonine Antikörper (HRP)
-
- Target Alle Phosphothreonine Produkte
- Phosphothreonine
- Reaktivität
- Bitte anfragen
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser Phosphothreonine Antikörper ist konjugiert mit HRP
-
Applikation
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Hersteller Produkt- Nr.
- 600-403-263
- Hersteller
- Rockland
- Verwendungszweck
- Phosphothreonine Antibody Peroxidase Conjugated
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Peroxidase.
- Produktmerkmale
- Synonyms: Phospho-Threonine Antibody, Phospho-Threonine ELISA, HRP Phospho Threonine (pT) antibody, PhosphoThreonine (pT) polyclonal antibody
- Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.
- Sterilität
- Sterile filtered
- Immunogen
-
Immunogen: Phospho Site Specific affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.
Immunogen Type: Other
- Isotyp
- IgG
-
-
- Applikationshinweise
-
Immunohistochemistry Dilution: User Optimized
Application Note: Phospho Site Specific Threonine affinity purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine proteins in both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Western Blot Dilution: 1:500
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:1,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 0.25 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: None - Konservierungsmittel
- Without preservative
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
-
-
PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system." in: Nature communications, Vol. 5, pp. 3527, (2014) (PubMed).
: "
-
PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system." in: Nature communications, Vol. 5, pp. 3527, (2014) (PubMed).
-
- Target
- Phosphothreonine
- Abstract
- Phosphothreonine Produkte
- Substanzklasse
- Amino Acid
- Hintergrund
- Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
-