Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Phosphothreonine Antikörper (HRP)

Rockland 600-403-263 WB, ELISA, IP, IHC Wirt: Kaninchen Polyclonal HRP
Rockland
Produktnummer ABIN5596878
Hersteller Produkt- Nr.: 600-403-263
  • Target Alle Phosphothreonine Produkte
    Phosphothreonine
    Reaktivität
    Bitte anfragen
    Wirt
    • 16
    • 6
    Kaninchen
    Klonalität
    • 15
    • 7
    Polyklonal
    Konjugat
    • 14
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser Phosphothreonine Antikörper ist konjugiert mit HRP
    Applikation
    • 22
    • 19
    • 16
    • 7
    • 6
    • 5
    • 4
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Hersteller Produkt- Nr.
    600-403-263
    Hersteller
    Rockland
    Verwendungszweck
    Phosphothreonine Antibody Peroxidase Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Peroxidase.
    Produktmerkmale
    Synonyms: Phospho-Threonine Antibody, Phospho-Threonine ELISA, HRP Phospho Threonine (pT) antibody, PhosphoThreonine (pT) polyclonal antibody
    Aufreinigung
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Phospho Site Specific affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

    Immunogen Type: Other

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Phospho Site Specific Threonine affinity purified antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues.  The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine proteins in both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

    Western Blot Dilution: 1:500

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:1,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.25 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: None
    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Puttagunta, Tedeschi, Sória, Hervera, Lindner, Rathore, Gaub, Joshi, Nguyen, Schmandke, Laskowski, Boutillier, Bradke, Di Giovanni: "PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system." in: Nature communications, Vol. 5, pp. 3527, (2014) (PubMed).

  • Target
    Phosphothreonine
    Abstract
    Phosphothreonine Produkte
    Substanzklasse
    Amino Acid
    Hintergrund
    Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
Sie sind hier:
Kundenservice