Amylase, alpha Antikörper (HRP)

Produktnummer ABIN5596904

Hersteller Produkt- Nr.: 200-4345-0100

Produktdetails anti-Amylase, alpha Antikörper (HRP)

Target: Amylase, alpha (AMY)

Reaktivität: Bacillus amyloliquefaciens

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Amylase, alpha Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 200-4345-0100

Hersteller: Rockland

Verwendungszweck: Alpha Amylase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].

Produktmerkmale: Synonyms: rabbit anti-Alpha Amylase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Aufreinigung: Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Alpha Amylase Antibody was produced by repeated immunizations with Bacillus amyloliquefaciens a-Amylase protein.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Alpha Amylase Peroxidase conjugate antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Alpha Amylase [Bacillus amyloliquefaciens] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Amylase, alpha

Target: Amylase, alpha (AMY)

Andere Bezeichnung: Alpha-amylase (AMY Produkte)

Hintergrund: Background: Anti-Alpha Amylase Antibody detects Alpha Amylase. α-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Anti-Alpha Amylase Antibody is ideal for investigators involved in Cell Signaling.

Gen-ID: 7849308

NCBI Accession: WP_013352208

UniProt: P00692