Amylase, alpha Antikörper (HRP)
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- Target Alle Amylase, alpha (AMY) Antikörper anzeigen
- Amylase, alpha (AMY)
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Reaktivität
- Bacillus amyloliquefaciens
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Amylase, alpha Antikörper ist konjugiert mit HRP
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Applikation
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Kreuzreaktivität (Details)
- Cross reactivity against a-Amylase from other tissues and species is unknown.
- Aufreinigung
- Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].
- Immunogen
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Immunogen: Anti-Alpha Amylase Antibody was produced by repeated immunizations with Bacillus amyloliquefaciens a-Amylase protein.
Immunogen Type: Native Protein
- Isotyp
- IgG
- Top Product
- Discover our top product AMY Primärantikörper
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- Applikationshinweise
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Application Note: Alpha Amylase antibody has been assayed against 1.0 μg of Alpha Amylase [Bacillus amyloliquefaciens] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Konservierungsmittel
- Gentamicin sulfate
- Vorsichtsmaßnahmen
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- Amylase, alpha (AMY)
- Andere Bezeichnung
- Alpha Amylase (AMY Produkte)
- Synonyme
- LOC663954 antikoerper, LOC100136478 antikoerper, alpha amylase antikoerper, alpha-amylase antikoerper, LOC663954 antikoerper, HQ_RS05305 antikoerper, LOC100136478 antikoerper
- Hintergrund
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Synonyms: Alpha-amylase, EC=3.2.1.1, 1,4-alpha-D-glucan glucanohydrolase
Background: Anti-Alpha Amylase Antibody detects Alpha Amylase. α-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Anti-Alpha Amylase Antibody is ideal for investigators involved in Cell Signaling.
Gene Name: Alpha-amylase
- Gen-ID
- 7849308
- UniProt
- P00692
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