Trypsin Antikörper (HRP)

Produktnummer ABIN5596916

Produktdetails anti-Trypsin Antikörper (HRP)

Target: Trypsin

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Trypsin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity against Trypsin from other sources is unknown.

Aufreinigung: Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas].

Immunogen:

Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Trypsin Antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:14,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Trypsin

Target: Trypsin

Abstract: Trypsin Produkte

Synonyme: wu:fb57g08 antikoerper, zgc:109701 antikoerper, LOC397853 antikoerper, Trypsin antikoerper, trp1 antikoerper, try1 antikoerper, trypsin antikoerper, protease, serine 1 antikoerper, protease, serine 1 L homeolog antikoerper, protease, serine, 1 antikoerper, trypsin antikoerper, prss1 antikoerper, prss1.L antikoerper, Deipr_2253 antikoerper

Hintergrund:

Synonyms: Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody

Background: Anti-Trypsin Antibody detects trypsin. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. It belongs to the peptidase S1 family. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. It cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. Trypsin hydrolyzes ester and amide linkages of several synthetic substrates. Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Biochemistry research.

Gene Name: TRBOTR

Gen-ID: 780933

UniProt: P00760