Pepsin A Antikörper (HRP)

Produktnummer ABIN5596959

Produktdetails anti-Pepsin A Antikörper (HRP)

Target: Pepsin A

Reaktivität: Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Pepsin A Antikörper ist konjugiert mit HRP

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Dot Blot (DB)

Verwendungszweck: Pepsin Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].

Produktmerkmale: Synonyms: goat anti-Pepsin Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody

Aufreinigung: Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Pepsin [Porcine Stomach]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:125 - 1:625

Application Note: Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.

Western Blot Dilution: 1:200 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Pepsin A

Target: Pepsin A

Andere Bezeichnung: PGA (Pepsin A Produkte)

Hintergrund: Background: Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab')2 fragment from antibodies.

Gen-ID: 396892

UniProt: P00791