R-Phycoerythrin Antikörper (Biotin)

Produktnummer ABIN5596964

Hersteller Produkt- Nr.: 600-106-387

Produktdetails anti-R-Phycoerythrin Antikörper (Biotin)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser R-Phycoerythrin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-106-387

Hersteller: Rockland

Verwendungszweck: R-Phycoerythrin Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, and R-Phycoerythrin conjugated IgG.

Produktmerkmale: Synonyms: goat anti R-Phycoerythrin Antibody biotin conjugate, goat anti Phycoerythrin biotin conjugate, goat anti-R-Phycoerythrin biotin conjugation

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: Anti-R-Phycoerythrin Antibody Biotin Conjugated has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA as well as other immunochemical assays.  R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.  Reaction with low molecular weight fragments is typically noted by western blot.

Western Blot Dilution: 1:2,000 - 1:20,000

ELISA Dilution: 1:200,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Andere Bezeichnung: R-Phycoerythrin (R-Phycoerythrin Produkte)

Substanzklasse: Chemical

Hintergrund: Background: Anti-R-Phycoerythrin Antibody Biotin Conjugated generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 240 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-R-PE antibody is conjugated to Biotin.