Protein A Antikörper (Biotin)

Produktnummer ABIN5596968

Produktdetails anti-Protein A Antikörper (Biotin)

Target: Protein A

Reaktivität: Staphylococcus aureus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Protein A Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity against Protein A from other sources is unknown.

Aufreinigung: Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].

Immunogen:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500-1:2,500

Application Note: Anti-Protein A Biotin Conjugated has been assayed against 1.0 μg of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:2,000-1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Protein A

Target: Protein A

Abstract: Protein A Produkte

Synonyme: alpha protein antikoerper, repB antikoerper

Hintergrund:

Synonyms: BAC, Protein A Biotin, Anti-Protein A BAC Antibody

Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein A antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.