TET2 Antikörper (N-Term)

Produktnummer ABIN5596979

Produktdetails anti-TET2 Antikörper

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TET2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): It is directed against, and shows specific reactivity for human Tet2 protein. Cross reactivity with Tet1 and Tet3 has not been determined.

Aufreinigung: Anti-TET2 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.

Immunogen:

Immunogen: TET2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-2 domain containing the N-terminal 156 amino acids of the protein.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Tet2 antibody has been tested for use by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~230 kDa and ~150 kDa (isoforms).

Western Blot Dilution: 1:5000

ELISA Dilution: 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 72.50 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TET2

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Andere Bezeichnung: TET2 (TET2 Produkte)

Synonyme: KIAA1546 antikoerper, MDS antikoerper, Ayu17-449 antikoerper, E130014J05Rik antikoerper, mKIAA1546 antikoerper, RGD1311625 antikoerper, tet methylcytosine dioxygenase 2 antikoerper, TET2 antikoerper, Tet2 antikoerper

Hintergrund:

Synonyms: Tet2 antibody, Methylcytosine dioxygenase TET2, KIAA1546 antibody, myeloproliferative disorder antibody, MPD antibody, hematopoietic cell antibody, TET2 antibody,Tet oncogene 2 antibody, MDS antibody, TET2_HUMAN antibody

Background: Tet2 antibody is a dioxygenase enzyme which is associated with the addition of the hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet2 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet2 plays an active role in catalyzing those conversions which have been suggested as the first step for active mammalian DNA de-methylation. Tet2 enzyme aids in the epigenetic modification of mammalian cystosine bases, ultimately affecting transcriptional regulation. Additionally methylcystosine dioxygenase Tet2 recruits O-GlcNAc transferase OGT enzyme to CpG-rich transcription start sites of the gene, encouraging H2B GlcNAcylation by OGT. Anti-TET2 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.

Gene Name: TET2

Gen-ID: 54790

UniProt: Q6N021

Pathways: Warburg Effekt