Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
In 1x PBS, pH 7.4
Handhabung
Aliquot to avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Fujita, Yamamoto, Imamura, Nakamura, Maehara, Tsuneyoshi, Oda: "Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach." in: Virchows Archiv : an international journal of pathology, Vol. 460, Issue 2, pp. 163-9, (2012) (PubMed).
Kashima, Idogawa, Mita, Shitashige, Yamada, Ogi, Suzuki, Toyota, Ariga, Sasaki, Tokino: "CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12975-84, (2012) (PubMed).
Hu, Huang, Sun, Wu, Xu, Yin, Chen, Jiang, Shen: "Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer." in: Medical oncology (Northwood, London, England), Vol. 28, Issue 2, pp. 447-54, (2011) (PubMed).
Privette, Weier, Nguyen, Yu, Petty: "Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 7, pp. 643-52, (2008) (PubMed).
Gao, Xin, Zhang, Zhou: "Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma." in: World journal of gastroenterology : WJG, Vol. 14, Issue 32, pp. 5000-7, (2008) (PubMed).
Milne, Sitarz, Carvalho, Polak, Ligtenberg, Pauwels, Offerhaus, Weterman: "Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis." in: Human pathology, Vol. 38, Issue 6, pp. 903-13, (2007) (PubMed).
Privette, González, Ding, Kleer, Petty: "Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression." in: Cancer research, Vol. 67, Issue 13, pp. 6064-74, (2007) (PubMed).
Target
CHFR
(Checkpoint with Forkhead and Ring Finger Domains (CHFR))