Nidogen 1 Antikörper

Produktnummer ABIN5662645

Produktdetails anti-Nidogen 1 Antikörper

Target: Nidogen 1 (NID1)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Nidogen 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Mouse.

Aufreinigung: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Immunogen: ELM1 is a rat monoclonal IgG2a antibody derived by fusion of X63 Ag 8.653 mouse myeloma cells with spleen cells from a Fisher rat immunized with a partially purified preparation of laminin from the EHS mouse tumor.

Klon: ELM1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELM1 reacts with entactin. APPLICATIONS ELM1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-Nidogen 1 Antikörper (ABIN5662645)

Couchman, Ljubimov: "Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies." in: Matrix (Stuttgart, Germany), Vol. 9, Issue 4, pp. 311-21, (1989) (PubMed).

Ljubimov, Afanasjeva, Litvinova, Senin: "Basement membrane components produced by a mouse ascites teratocarcinoma TB24. Analysis with monoclonal and polyclonal antibodies." in: Experimental cell research, Vol. 165, Issue 2, pp. 530-40, (1986) (PubMed).

Details zu Nidogen 1

Target: Nidogen 1 (NID1)

Andere Bezeichnung: Entactin / Nidogen (NID1 Produkte)

Hintergrund: Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulphated glycoprotein of approximately 150 kD.