Rekombinanter Fluorescein Antikörper

Produktnummer ABIN5668030

Produktdetails

Target: Fluorescein

Antikörpertyp: Recombinant Antibody

Wirt: Human

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: In vivo Studies (in vivo), Negative Control (NC)

Verwendungszweck: Anti-Fluorescein [4-4-20 (enhanced)], Human IgG1, kappa

Spezifität: This antibody is specific for fluorescein I.

Kreuzreaktivität: Diverse Spezies

Produktmerkmale:

Original Species of Ab: Human

Original Format of Ab: scFv

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).

Klon: 4-4-20 (enhanced)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999, PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein.

Kommentare:

This is a reformatted antibody made using the variable domain sequences of the original human scFv format to improve compatibility with existing reagents, assays, and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Referenzen

Finlay, Coleman, Edwards, Johnson: "Anti-PD1 'SHR-1210' aberrantly targets pro-angiogenic receptors and this polyspecificity can be ablated by paratope refinement." in: mAbs, Vol. 11, Issue 1, pp. 26-44, (2019) (PubMed).

Antigendetails

Target: Fluorescein

Substanzklasse: Chemical

Hintergrund: FL, FlI, FITC, Fluorescein isothiocyanate, fluorescein isothiocyanate, isomer 1, Fluorescyl, Resorcinolphthalein, C.I. 45350, solvent yellow 94, D & C yellow no. 7, Angiofluor, Japan yellow 201, Soap yellow, 3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one