Rekombinanter RTN4RL2 Antikörper (AA 279-420)

Produktnummer ABIN5668205

Produktdetails anti-RTN4RL2 Antikörper

Target: RTN4RL2 (Reticulon 4 Receptor-Like 2 (RTN4RL2))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 279-420

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RTN4RL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Verwendungszweck: Anti-NgR2 [P14], Rabbit IgG, kappa

Spezifität: The antibody binds to human NgR2 with a Kd of 2.3 nM and to rat NgR2 with a Kd of 0.48 nM (bivalent binding). The antibody binds specifically to residues 351-399 of NgR2, a member of the Nogo-66 receptor family which inhibits axonal growth and sporulating in the CNS.

Kreuzreaktivität: Human, Ratte

Produktmerkmale:

Original Species of Ab: Rabbit

Original Format of Ab: IgG

Aufreinigung: Protein A affinity purified

Immunogen: (His)6-tagged fragment of rat NgR2 (amino acids 279-420).

Klon: P14

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The original antibody was used for for an ELISA on the NgR2 antigen to confirm the binding of the antibody. This antibody was used for a western blot on lysates of 293F cells transfected with vectors for mammalian cell expression of human NgR1, NgR2, and NgR3. This antibody was used for flow cytometry on 293F cells transfected with human NgR1, NgR2, and NgR3 mammalian cell expression vectors. This antibody was used for surface plasmon resonance on NgR1 and NgR2 to determine its binding affinity for its antigen. The antibody was used for immunofluorescence on PC12 cells and PC12-NgR1 or PC12-NgR2. This antibody was used for immunoprecipitation on rat PC12 cell lines stably expressing full-length rat NgR1 or NgR2 (Hofer et al., 2008, PMID:17140598).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu RTN4RL2

Target: RTN4RL2 (Reticulon 4 Receptor-Like 2 (RTN4RL2))

Andere Bezeichnung: NgR2 (RTN4RL2 Produkte)

Hintergrund: Nogo-66 Receptor

UniProt: Q86UN3