Immunohistochemistry (IHC), Western Blotting (WB), ELISA
Kreuzreaktivität (Details)
human TGFalpha 100%. (Neutralizing antibody)We found no crossreactivty to other cytokines such as hTGFß, hEGF, hPDGf, hTNF. hEPO, hIGF, 1mg per ml. hIL-1, hIL-2, hIL-3, hIL-4, hIL-5, hIL-6, hIL-7, hIL-8, hIL-9, hIL-10, hIL-11,and hIL-12
Aufreinigung
Purified
Endotoxin-Niveau
<0.1 EU per 1 µg of the antibody as determined by the LAL method.
Immunogen
TGF alpha antibody was raised in goat using E. coli-derived recombinant human TGF-alpha as the immunogen.
ELISA: 2-8 µg/mL, IHC: 15 µg/mL, WB: 1 µg/mL Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with PBS buffer.
Konzentration
Lot specific
Buffer
Supplied as a lyophilized powder.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
Transforming growth factor alpha (TGF- alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGF alpha stimulates neural cell proliferation in the adult injured brain.