Dieser Glucuronidase beta Antikörper ist konjugiert mit Biotin
Applikation
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)
Spezifität
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Produktmerkmale
Molar Ratio: Biotin/IgG~ 6.2
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogen
Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
GUSB
Reaktivität: Human
WB, IHC, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore by adding 1.0 mL of sterile distilled water.
Konzentration
10 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB