Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Radioimmunoassay (RIA), Dot Blot (DB), Immunodiffusion (ID)
Spezifität
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes Alcohol Dehydrogenase.
Produktmerkmale
Molar Ratio: ~5.5
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogen
Alcohol dehydrogenase isolated and purified from Baker's Yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore by adding 1.0 mL of sterile distilled water.
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.Synonyms: ADC1, ADH1, Alcohol dehydrogenase I, O0947, YADH-1, YOL086C