Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
Produktmerkmale
Molar Ratio: Biotin/IgG= ~5.4
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogen
L-Lactic dehydrogenase, LDH-5(M4) isoenzyme is isolated and purified from Human placenta. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.Synonyms: L-Lactic dehydrogenase LDH-5 Isozyme M4