Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA)
Spezifität
IgG fraction of polyclonal rabbit antiserum to succinic thiokinase from porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Succinic thiokinase is isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.