Ficin Antikörper
Reaktivität: Fig
IF, WB, DB, EIA, ID, IP, RIA
Wirt: Kaninchen
Polyclonal
unconjugated
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- Target Alle Ficin Produkte
- Ficin
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Reaktivität
- Fig
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Ficin Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
- Aufreinigung
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- Immunogen
- Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotyp
- IgG
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore by adding 1.0 mL of sterile distilled water
- Konzentration
- 10.0 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
- Konservierungsmittel
- Without preservative
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Target
- Ficin
- Abstract
- Ficin Produkte
- Hintergrund
- Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)
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