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Ficin Antikörper (Biotin)

Reaktivität: Fig IF, WB, DB, EIA, ID, IP, RIA Wirt: Kaninchen Polyclonal Biotin
Produktnummer ABIN568456
  • Target Alle Ficin Produkte
    Ficin
    Reaktivität
    • 3
    • 2
    Fig
    Wirt
    • 5
    Kaninchen
    Klonalität
    • 5
    Polyklonal
    Konjugat
    • 3
    • 2
    Dieser Ficin Antikörper ist konjugiert mit Biotin
    Applikation
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
    Produktmerkmale
    Molar Ratio: Biotin/IgG ~ 6.0
    Aufreinigung
    Ammonium Sulphate Precipitation and Ion Exchange Chromatography
    Immunogen
    Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure
    Isotyp
    IgG
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    Restore by adding 1.0 mL of sterile distilled water
    Konzentration
    10.0 mg/mL
    Buffer
    PBS, pH 7.2 without preservatives and foreign proteins
    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target
    Ficin
    Abstract
    Ficin Produkte
    Hintergrund
    Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)
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