In Immunoelectrophoresis against Human serum a single precipitin line is obtained. The antiserum does not react with any other protein component of Human serum or plasma. The antiserum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE-Chromatography
Immunogen
The antigen has been isolated from pooled Human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/mL.Synonyms: CFP, Complement factor P, PFC