FGF acidic Antikörper

Produktnummer ABIN5688992

Produktdetails

Target: FGF acidic

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Aufreinigung: Anti-hFGF-acidic specific antibody was purified by affinity chromatography employing immobilized hFGF-acidic matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-acidic (human Fibroblast Growth Factor-acidic).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-acidic (30.0 ng/mL), a concentration of 0.46 - 0.9 μ,g/mL of this antibody is required.

ELISA:

To detect hFGF-acidic by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-acidic.

Sandwich: To detect hFGF-acidic by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-acidic (XP-5136Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-acidic.

Western Blot:

To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: FGF-acidic antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: FGF acidic

Andere Bezeichnung: FGF-acidic

Gen-ID: 2246

UniProt: P05230