Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist, this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.
Aufreinigung
DNAJC13 antibody is affinity chromatography purified via peptide column.
Immunogen
DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.
The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
DNAJC13 antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
DNAJC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
DNAJC13
(DnaJ heat shock protein family (Hsp40) member C13 (DNAJC13))
RME8 antikoerper, D030002L11Rik antikoerper, Gm1124 antikoerper, RME-8 antikoerper, Rme8 antikoerper, mKIAA0678 antikoerper, DnaJ heat shock protein family (Hsp40) member C13 antikoerper, dnaJ homolog subfamily C member 13 antikoerper, DNAJC13 antikoerper, LOC100167963 antikoerper, LOC100640734 antikoerper, Dnajc13 antikoerper
Hintergrund
DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).