Dieser Heregulin beta 1 Antikörper ist unkonjugiert
Applikation
ELISA, Western Blotting (WB), Neutralization (Neut)
Immunogen
Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin-B1. Human Heregulin-B1 specific antibody was purified by affinity chromatography employing an immobilized Human Heregulin-B1 matrix.
Reaktivität: Human
ELISA, WB
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-B1.
Western Blot: To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Lagerung
-20 °C
Informationen zur Lagerung
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.