Dieser Heregulin beta 1 Antikörper ist konjugiert mit Biotin
Applikation
ELISA, Western Blotting (WB)
Immunogen
Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin- β,1. Human Heregulin- β,1 specific antibody was purified by affinity chromatography and then biotinylated.
Reaktivität: Human
ELISA, WB, Neut
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Direct: To detect hHeregulin-β,1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-β,1.
Sandwich To detect hHeregulin-β,1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Heregulin-β,1 (38-244) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-β,1.
Western Blot: To detect hHeregulin-β,1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-β,1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Lagerung
-20 °C
Informationen zur Lagerung
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.