NSF Antikörper (AA 620-744)

Produktnummer ABIN5693126

Produktdetails anti-NSF Antikörper

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Bindungsspezifität: AA 620-744

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Marke: Picoband™

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for NSF detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human NSF recombinant protein (Position: N620-D744).

Anwendungsinformationen

Applikationshinweise:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Details zu NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Andere Bezeichnung: NSF (NSF Produkte)

Synonyme: SKD2 antikoerper, AI316878 antikoerper, AU020090 antikoerper, AU067812 antikoerper, NSF antikoerper, 18.m06598 antikoerper, nsf antikoerper, si:bz18k17.1 antikoerper, wu:fj33g11 antikoerper, N-ethylmaleimide sensitive factor antikoerper, T1J1.4 antikoerper, T1J1_4 antikoerper, NEM-sensitive fusion protein antikoerper, N-ethylmaleimide sensitive factor, vesicle fusing ATPase antikoerper, N-ethylmaleimide sensitive fusion protein antikoerper, N-ethylmaleimide-sensitive factor antikoerper, N-ethylmaleimide sensitive factor antikoerper, N-ethylmaleimide-sensitive fusion protein, putative antikoerper, N-ethylmaleimide sensitive factor L homeolog antikoerper, vesicle-fusing ATPase antikoerper, N-ethylmaleimide-sensitive factor a antikoerper, AAA-type ATPase family protein antikoerper, NSF antikoerper, Nsf antikoerper, nsf antikoerper, BBOV_II007200 antikoerper, TGME49_318510 antikoerper, nsf.L antikoerper, LOC100180685 antikoerper, LOC9310886 antikoerper, nsfa antikoerper

Hintergrund:

Synonyms: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF

Background: N-ethylmaleimide-sensitive factor, also known as NSF, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.

UniProt: P46459