CD8 alpha Antikörper (AA 28-196)

Produktnummer ABIN5693154

Produktdetails anti-CD8 alpha Antikörper

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Bindungsspezifität: AA 28-196

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD8 alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Marke: Picoband™

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for CD8 alpha detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Mouse,Rat.

Immunogen: E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196).

Anwendungsinformationen

Applikationshinweise:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry(Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry, 1-3 μg/1x10⁶ cells
Direct ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-CD8 alpha Antikörper (ABIN5693154)

Xu, Tan, Wang, Wang, Li, Wu, Chen, Wu, Tan: "Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion." in: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1501-10, (2015) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: Cd8a (CD8A Produkte)

Synonyme: cd8 antikoerper, LOC100136450 antikoerper, BB154331 antikoerper, Ly-2 antikoerper, Ly-35 antikoerper, Ly-B antikoerper, Lyt-2 antikoerper, zgc:136643 antikoerper, CD8 antikoerper, Leu2 antikoerper, MAL antikoerper, p32 antikoerper, RHACD8-4 antikoerper, CD8a molecule antikoerper, uncharacterized LOC100125537 antikoerper, CD8 alpha antikoerper, CD8 antigen, alpha chain antikoerper, T-cell surface glycoprotein CD8 alpha chain antikoerper, Cd8a antikoerper, LOC100125537 antikoerper, LOC100136450 antikoerper, CD8A antikoerper, cd8a antikoerper, LOC100126556 antikoerper

Hintergrund:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

UniProt: P01731

Pathways: T-Zell Rezeptor Signalweg