Histone 3 Antikörper (H3K27me3, pSer28)
(1 reference)
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- Target Alle Histone 3 (H3) Antikörper anzeigen
- Histone 3 (H3)
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Bindungsspezifität
- H3K27me3, pSer28
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Reaktivität
- Human, Maus, C. elegans
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Histone 3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)
- Hersteller Produkt- Nr.
- 600-401-i84
- Hersteller
- Rockland
- Verwendungszweck
- Histone H3 K27me3/phospho S28 Antibody
- Kreuzreaktivität (Details)
- This antibody reacts with human Histone H3.
- Produktmerkmale
- Synonyms: rabbit anti-Histone H3 trimethyl Lys18 pS28 antibody, H3.3B, H3pS28K18Me3, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3 K27me3/pS28
- Aufreinigung
- Anti-Histone H3 [Trimethyl Lys27, p Ser28] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Histone H3 [Trimethyl Lys27, p Ser28] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic trimethylated/phosphorylated peptides surrounding Lysine 27 and Serine 28 of human Histone H3.
Immunogen Type: Conjugated Peptide
- Isotyp
- IgG
- Top Product
- Discover our top product H3 Primärantikörper
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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reaktivität: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 Reaktivität: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 Reaktivität: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 Reaktivität: Human, Maus WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Wirt: Maus Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 Reaktivität: Human, Maus WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 Reaktivität: Human, Maus, Ratte WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 Reaktivität: Human WB, IF, IHC, ChIP, IP, ChIP-seq Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reaktivität: Human, Maus, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 Reaktivität: Human WB, IHC, ChIP, IP Wirt: Kaninchen Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reaktivität: Human WB, IF, IHC, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Wirt: Kaninchen Polyclonal unconjugated
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:2000
Application Note: Anti-Histone H3 [Trimethyl Lys27, p Ser28] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1 μg/mL
IF Microscopy Dilution: 1:2000
Other: Dot Blot 1 μg/mL
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.66 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.05 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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-
: "Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma." in: Journal of clinical medicine, Vol. 8, Issue 3, (2019) (PubMed).
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: "Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma." in: Journal of clinical medicine, Vol. 8, Issue 3, (2019) (PubMed).
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- Target
- Histone 3 (H3)
- Andere Bezeichnung
- Histone H3 (H3 Produkte)
- Hintergrund
- Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, trimethylation of K27 is associated with gene silencing, whereas pS28 is associated with mitosis and immediate early genes. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
- Gen-ID
- 126961
- NCBI Accession
- NP_001005464
- UniProt
- Q71DI3
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