Histone 3 Antikörper (H3K9me)

Produktnummer ABIN5706783

Hersteller Produkt- Nr.: 600-401-i69

Produktdetails anti-Histone 3 Antikörper

Target: Histone 3 (H3)

Bindungsspezifität: H3K9me

Reaktivität: Human, Maus, C. elegans

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-i69

Hersteller: Rockland

Verwendungszweck: Histone H3 K9me1 Antibody

Kreuzreaktivität (Details): This antibody reacts with human Histone H3.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 monomethyl Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9me1

Aufreinigung: Anti-Histone H3 [Monomethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [Monomethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 9 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Histone H3 [Monomethyl Lys9] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:500

Other: Dot Blot 0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.7 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3 (H3 Produkte)

Hintergrund: Background: Histone H3 K9 methylation is a conserved epigenetic silencer of transcription. However, for this modification to make its effect, the G9a/GLP heteromeric complex is required to methylate histone H3. Throughout development of normal tissues, DNA methylation and stable gene silencing is required to optimize cellular development. Likewise, in cancer, dysfunctions in these normal functions are required to enhance cellular proliferation. When K3K9 me1 silences the expression of RIZ1, normal apoptosis of pre-cancerous cells does not occur, and proliferation goes ahead unabated. From H3K9me1, conversion to H3K9me3 is mediated by SUVR4, at transposons and pseudogenes. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gen-ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3