Ngp Antikörper (C-Term)

Produktnummer ABIN5706813

Produktdetails anti-Ngp Antikörper

Target: Ngp (NGP) (Neutrophilic Granule Protein (NGP))

Bindungsspezifität: C-Term

Reaktivität: Maus, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Fluorescence Microscopy (FM), Western Blotting (WB)

Produktmerkmale:

Synonyms: rabbit anti-Ngp antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein

Background: This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.

Gene Name: Ngp

Aufreinigung: Anti-Ngp is directed against full-length mouse Ngp. This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in Ngp based on 100% homology match for the immunogen sequence.

Immunogen:

Immunogen: Affinity purified Anti-Ngp antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse Ngp protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in IF, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to Ngp by western blotting in the appropriate cell lysate or extract. 

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 12 months

Referenzen für anti-Ngp Antikörper (ABIN5706813)

Hong, Qu, Wuest, Huang, Huang, Lin: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).

Details zu Ngp

Target: Ngp (NGP) (Neutrophilic Granule Protein (NGP))

Andere Bezeichnung: Ngp

Synonyme: NGP antikoerper, bectenecin antikoerper, neutrophilic granule protein antikoerper, Ngp antikoerper

Gen-ID: 18054

NCBI Accession: NP_032720