F11R Antikörper (C-Term, pTyr280)

Produktnummer ABIN5706868

Produktdetails anti-F11R Antikörper

Target: F11R (F11 Receptor (F11R))

Bindungsspezifität: C-Term, pTyr280

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser F11R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: rabbit anti-JAM A pY280 antibody, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA

Background: Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.

Gene Name: F11R

Aufreinigung: Anti-JAM A pY280 is directed against human JAM A phosphorylated at the tyrosine 280 position.    This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.  A BLAST analysis was used to suggest reactivity with this protein from human and feline based on 100% homology for the immunogen sequence.

Immunogen:

Immunogen: Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA, IF, and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 5.0 μg/mL

IF Microscopy Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 12 months

Referenzen für anti-F11R Antikörper (ABIN5706868)

Tapia, Kralicek, Hecht: "Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells." in: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).

Fan, Weight, Luissint, Hilgarth, Brazil, Ettel, Nusrat, Parkos: "Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation." in: Molecular biology of the cell, Vol. 30, Issue 5, pp. 566-578, (2019) (PubMed).

Details zu F11R

Target: F11R (F11 Receptor (F11R))

Andere Bezeichnung: JAM A (F11R Produkte)

Synonyme: CD321 antikoerper, JAM antikoerper, JAM1 antikoerper, JAMA antikoerper, JCAM antikoerper, KAT antikoerper, PAM-1 antikoerper, 9130004G24 antikoerper, AA638916 antikoerper, ESTM33 antikoerper, JAM-1 antikoerper, JAM-A antikoerper, Jcam antikoerper, Jcam1 antikoerper, Ly106 antikoerper, Jam1 antikoerper, F11 receptor antikoerper, F11R antikoerper, F11r antikoerper

Gen-ID: 50848

NCBI Accession: NP_058642

Pathways: Cell-Cell Junction Organization