E2F8 Antikörper (Internal Region)

Produktnummer ABIN570707

Produktdetails anti-E2F8 Antikörper

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser E2F8 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: E2F8

Sequenz: KSIESDRRKI NSAPS

Kreuzreaktivität: Rind (Kuh), Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-KSIESDRRKINSAPS , from the internal region of the protein sequence according to NP_078956.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 80 kDa in Human K562 and Mouse Neuro2a cell lysate after 0.3 μg/mL antibody staining. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu E2F8

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Andere Bezeichnung: E2F8 (E2F8 Produkte)

Synonyme: MGC130750 antikoerper, E2F8 antikoerper, E2F-8 antikoerper, RGD1308091 antikoerper, 4432406C08Rik antikoerper, AA410048 antikoerper, E2F transcription factor 8 antikoerper, E2F transcription factor 8 L homeolog antikoerper, E2F8 antikoerper, e2f8.L antikoerper, e2f8 antikoerper, E2f8 antikoerper

Hintergrund: E2F8, E2F transcription factor 8, FLJ23311, E2F family member 8

Gen-ID: 79733, 108961

NCBI Accession: NP_078956