The stated application concentrations are suggested starting points. Titration of the recombinant Napsin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Konservierungsmittel
Azide free
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store the recombinant Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
KAP antikoerper, Kdap antikoerper, NAP1 antikoerper, NAPA antikoerper, SNAPA antikoerper, pronapsin antikoerper, kap antikoerper, kdap antikoerper, nap1 antikoerper, napa antikoerper, napsa antikoerper, snapa antikoerper, napsin A aspartic peptidase antikoerper, napsin A aspartic peptidase L homeolog antikoerper, NAPSA antikoerper, Napsa antikoerper, napsa.L antikoerper
Hintergrund
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.