Desmoplakin Antikörper (Internal Region)

Produktnummer ABIN570871

Produktdetails anti-Desmoplakin Antikörper

Target: Desmoplakin (DSP)

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Desmoplakin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Verwendungszweck: Desmoplakin (aa 1672 -1684)

Sequenz: QESVKQAHLR NEH

Spezifität: This antibody is expected to recognize reported isoform I (NP_004406.2) only.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: C-QESVKQAHLRNEH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been

Peptide ELISA: antibody detection limit dilution 1:4000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Desmoplakin

Target: Desmoplakin (DSP)

Andere Bezeichnung: DSP (DSP Produkte)

Hintergrund: DSP, desmoplakin, DPI, 250 kDa paraneoplastic pemphigus antigen, desmoplakin I

Gen-ID: 1832, 109620, 306871

NCBI Accession: NP_004406

Pathways: Cell-Cell Junction Organization