SPRY1 Antikörper (C-Term)
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- Target Alle SPRY1 Antikörper anzeigen
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser SPRY1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Sprouty
- Sequenz
- CPSRGQGKPS
- Spezifität
- NP_005832.1 and NP_955359.1 represent varients of the same protein.
- Kreuzreaktivität
- Human
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.
- Isotyp
- IgG
- Top Product
- Discover our top product SPRY1 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.
Western Blot: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS
Peptide ELISA: antibody detection limit dilution 1:16000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Rec
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
- Andere Bezeichnung
- SPRY1 (SPRY1 Produkte)
- Synonyme
- hSPRY1 antikoerper, SPRY1 antikoerper, LOC100223875 antikoerper, hspry1 antikoerper, xspry1 antikoerper, sprouty antikoerper, sprouty1 antikoerper, sprouty-1 antikoerper, Xtsprouty1 antikoerper, spry1a antikoerper, spry1b antikoerper, sprouty RTK signaling antagonist 1 antikoerper, sprouty RTK signaling antagonist 1 L homeolog antikoerper, sprouty RTK signaling antagonist 1 S homeolog antikoerper, sprouty homolog 1 (Drosophila) antikoerper, SPRY1 antikoerper, Spry1 antikoerper, spry1 antikoerper, spry1.L antikoerper, spry1.S antikoerper
- Hintergrund
- SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)
- Gen-ID
- 10252
- NCBI Accession
- NP_005832, NP_955359
- Pathways
- EGFR Signaling Pathway, EGFR Downregulation
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