PRDM1 Antikörper (Internal Region)
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- Target Alle PRDM1 Antikörper anzeigen
- PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser PRDM1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- PRDM1 / MEL1
- Sequenz
- DISDNADRLE DVED
- Spezifität
- This antibody is expected to recognize both reported isoforms (NP_001189.2, NP_878911.1).
- Kreuzreaktivität
- Rind (Kuh), Hund, Human, Maus, Schwein, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2, NP_878911.1.
- Isotyp
- IgG
- Top Product
- Discover our top product PRDM1 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 6 μg/mL.
Western Blot: Approx 90 kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100 kDa in lysates of cell line K562. An additional faint band of approx 70 kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8 kDa accor
Peptide ELISA: antibody detection limit dilution 1:1000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))
- Andere Bezeichnung
- PRDM1 (PRDM1 Produkte)
- Hintergrund
- PRDM1, PR domain containing 1, with ZNF domain, BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1, B-lymphocyte-induced maturation protein 1, OTTHUMP00000016918, PR-domain zinc finger protein 1, PRDI-binding factor-1, beta-interferon gene posit
- Gen-ID
- 639, 12142, 309871
- NCBI Accession
- NP_001189, NP_878911
- Pathways
- Regulation of Muscle Cell Differentiation
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