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Tppp Antikörper (Internal Region)

Verified Tppp Reaktivität: Human, Maus, Ratte WB, ELISA, IHC Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN571099
  • Target Alle Tppp Antikörper anzeigen
    Tppp (Tubulin Polymerization Promoting Protein (Tppp))
    Bindungsspezifität
    • 15
    • 7
    • 7
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 34
    • 33
    • 16
    • 7
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    Human, Maus, Ratte
    Wirt
    • 48
    • 3
    Ziege
    Klonalität
    • 49
    • 2
    Polyklonal
    Konjugat
    • 25
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Tppp Antikörper ist unkonjugiert
    Applikation
    • 44
    • 18
    • 13
    • 13
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Verwendungszweck
    TPPP/p25
    Sequenz
    DPSKDRAAKR LSLES
    Kreuzreaktivität
    Human
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    C-DPSKDRAAKRLSLES
    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Cortex. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7 kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Kommentare

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Tppp (Tubulin Polymerization Promoting Protein (Tppp))
    Andere Bezeichnung
    TPPP (Tppp Produkte)
    Hintergrund
    TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein
    Gen-ID
    11076, 72948, 361466
    NCBI Accession
    NP_008961
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