AIM2 Antikörper (C-Term)
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- Target Alle AIM2 Antikörper anzeigen
- AIM2 (Absent in Melanoma 2 (AIM2))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser AIM2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- AIM2
- Sequenz
- GEKLQLTSGV HSTIK
- Kreuzreaktivität
- Human
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.
- Isotyp
- IgG
- Top Product
- Discover our top product AIM2 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6 μg/mL.
Western Blot: Approx 37 kDa band observed in lysates of cell line HeLa (calculated MW of 39.0 kDa according to Human NP_004824.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:8000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- AIM2 (Absent in Melanoma 2 (AIM2))
- Andere Bezeichnung
- AIM2 (AIM2 Produkte)
- Synonyme
- AIM2 antikoerper, PYHIN4 antikoerper, Gm1313 antikoerper, Ifi210 antikoerper, absent in melanoma 2 antikoerper, AIM2 antikoerper, Aim2 antikoerper
- Hintergrund
- AIM2, absent in melanoma 2, PYHIN4
- Molekulargewicht
- 39.0kDa
- Gen-ID
- 9447
- NCBI Accession
- NP_004824
- Pathways
- Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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