POU3F3 Antikörper (Internal Region)
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- Target Alle POU3F3 Antikörper anzeigen
- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser POU3F3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- POU3F3 / BRN1 / OCT8
- Sequenz
- HMLSHAHQWV TAL
- Kreuzreaktivität
- Human, Maus, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.
- Isotyp
- IgG
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- Applikationshinweise
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Western Blot: Approx 48 kDa band observed in Mouse Spinal Cord lysates and approx. 50 kDa in Mouse Brain lysates (calculated MW of 50.3 kDa according to Human NP_006227.1 and 50.2 kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/mL. Pri
Peptide ELISA: antibody detection limit dilution 1:4000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
- Andere Bezeichnung
- POU3F3 (POU3F3 Produkte)
- Synonyme
- BRN1 antikoerper, OTF8 antikoerper, Brn1 antikoerper, RHS1 antikoerper, Brn-1 antikoerper, Otf8 antikoerper, Skin1 antikoerper, brn1 antikoerper, nrl-20 antikoerper, nrl20-A antikoerper, pou1 antikoerper, pou23 antikoerper, wu:fb92g06 antikoerper, zfpou1 antikoerper, zp23 antikoerper, zp23pou antikoerper, BRN-1.1 antikoerper, ZP12POU antikoerper, brn1.1 antikoerper, pou12 antikoerper, wu:fc33a11 antikoerper, zp12 antikoerper, POU class 3 homeobox 3 antikoerper, POU domain, class 3, transcription factor 3 antikoerper, POU class 3 homeobox 3 S homeolog antikoerper, POU class 3 homeobox 3b antikoerper, POU class 3 homeobox 3a antikoerper, POU3F3 antikoerper, Pou3f3 antikoerper, pou3f3.S antikoerper, pou3f3b antikoerper, pou3f3a antikoerper
- Hintergrund
- POU3F3, POU class 3 homeobox 3, BRN1, OTF8, OTF-8, POU domain, class 3, transcription factor 3, brain-1, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8
- Gen-ID
- 5455, 18993, 192109
- NCBI Accession
- NP_006227
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