Dystroglycan Antikörper (Internal Region)
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- Target Alle Dystroglycan (DAG1) Antikörper anzeigen
- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser Dystroglycan Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- DAG1
- Sequenz
- HVGKHEYFMH ATDK
- Spezifität
- This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
- Kreuzreaktivität
- Human
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- C-HVGKHEYFMHATDK
- Isotyp
- IgG
- Top Product
- Discover our top product DAG1 Primärantikörper
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anti-Dystroglycan 1 (DAG1) (AA 718-747), (C-Term) antibody
DAG1 Reaktivität: Human, Maus WB, IHC (p) Wirt: Kaninchen Polyclonal RB33752 unconjugated
anti-Dystroglycan 1 (DAG1) antibodyDAG1 Reaktivität: Human WB, ICC, IHC (p), IF Wirt: Kaninchen Polyclonal unconjugated
anti-Dystroglycan 1 (DAG1) (AA 28-406) antibodyDAG1 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Dystroglycan 1 (DAG1) (AA 31-140) antibodyDAG1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 2A3 unconjugated
anti-Dystroglycan 1 (DAG1) (AA 726-744) antibodyDAG1 Reaktivität: Human ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
anti-Dystroglycan 1 (DAG1) (C-Term) antibodyDAG1 Reaktivität: Human, Maus WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
anti-Dystroglycan 1 (DAG1) (AA 654-749) antibodyDAG1 Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated
anti-Dystroglycan 1 (DAG1) (AA 323-372) antibodyDAG1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Pferd, Affe, Fledermaus, Huhn, Schwein, Zebrafisch (Danio rerio) WB Wirt: Kaninchen Polyclonal unconjugated
anti-Dystroglycan 1 (DAG1) (AA 721-770) antibodyDAG1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Affe, Meerschweinchen, Fledermaus, Huhn WB Wirt: Kaninchen Polyclonal unconjugated
anti-Dystroglycan 1 (DAG1) (C-Term) antibodyDAG1 Reaktivität: Human IHC, IHC (fro) Wirt: Maus Monoclonal 43DAG1-8D5 unconjugated
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- Applikationshinweise
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 100 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4 kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
- Andere Bezeichnung
- DAG1 (DAG1 Produkte)
- Hintergrund
- DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1
- Gen-ID
- 1605
- NCBI Accession
- NP_004384
- Pathways
- Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
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