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Vinculin Antikörper

VCL Reaktivität: Human WB, IHC, IF, IP, IHC (p) Wirt: Maus Monoclonal unconjugated
Produktnummer ABIN604849
  • Target Alle Vinculin (VCL) Antikörper anzeigen
    Vinculin (VCL)
    Reaktivität
    • 116
    • 77
    • 67
    • 27
    • 18
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 89
    • 28
    • 1
    Maus
    Klonalität
    • 78
    • 40
    Monoklonal
    Konjugat
    • 79
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Vinculin Antikörper ist unkonjugiert
    Applikation
    • 90
    • 33
    • 30
    • 27
    • 23
    • 19
    • 15
    • 14
    • 13
    • 10
    • 10
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Recognizes human vinculin.
    Aufreinigung
    Protein G purified
    Immunogen
    Semi-purified vinculin. MW of Antigen: 130kD (vinculin) and 150kD (meta-vinculin). Cellular Localization: Cell membrane and cytoplasmic.

    Type of Immunogen: Protein extract
    Isotyp
    IgG1
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  • Applikationshinweise
    Approved: IF, IHC, IHC-P (2.5 - 5 μg/mL), IP, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Studies of vinculin in platelet activation. Immunoprecipitation (Denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2hrs at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS,  pH 7.4. No preservatives or stabilizing proteins added.
    Konservierungsmittel
    Azide free
    Handhabung
    avoid freeze thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Target
    Vinculin (VCL)
    Andere Bezeichnung
    VCL / Vinculin (VCL Produkte)
    Hintergrund
    Name/Gene ID: VCL
    Family: Cytoskeleton

    Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL
    Gen-ID
    7414
    UniProt
    P18206
    Pathways
    Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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