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CD19 Antikörper (AA 540-557)

CD19 Reaktivität: Human FACS, WB, IHC, ELISA, IP, IHC (p) Wirt: Maus Monoclonal unconjugated

Produktnummer ABIN609293

Datenblatt (PDF)

Target Alle CD19 Antikörper anzeigen

CD19 (CD19 Molecule (CD19))
Bindungsspezifität
23

16

16

14

10

7

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 540-557
Reaktivität
292

166

41

29

19

10

7

5

4

3

2

2

2

2

2

2

2

2
Human
Wirt
184

130

57

4
Maus
Passende Sekundärantikörper
Klonalität
256

115

2
Monoklonal
Konjugat
126

25

22

22

19

10

9

8

8

7

5

5

5

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
Dieser CD19 Antikörper ist unkonjugiert
Applikation
264

116

74

69

51

51

22

21

14

14

10

10

9

5

4

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität

Recognizes the C-terminal cytoplasmic tail sequence (CGPDPAWGGGGRMGTWSTR) of human CD19, a 95kD cell surface glycoprotein, which is expressed by cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. Detects a band of ~95kD in Raji cell lysates under reducing conditions.

Homologie

Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (94%) Elephant, Panda, Dog (89%) Pig (83%).

Aufreinigung

Protein G purified

Immunogen

CD19 peptide C-GPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (94%), Elephant, Panda, Dog (89%), Pig (83%).

Isotyp

IgG1

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CD19 Reaktivität: Human FACS, WB, IHC, IF, ELISA Wirt: Kaninchen Polyclonal unconjugated

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CD19 Reaktivität: Human FACS, IP Wirt: Maus Monoclonal LT19 unconjugated

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CD19 Reaktivität: Human FACS, ICC Wirt: Maus Monoclonal 4G7 unconjugated

Applikationshinweise

Approved: ELISA, Flo (1:100 - 1:200), IHC, IHC-P (1:100 - 1:200), IP, WB (1:100 - 1:200)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry (paraffin), Immunoprecipitation, and Flow Cytometry. Flow Cytometry: 1:100-1:200, 10 μL labels 10e6 cells in 100 μLImmunohistochemistry (paraffin): 1:100-1:200. Membrane permeabilization and antigen retrieval using heat treatment methods prior to staining of paraffin sections.

Kommentare

Target Species of Antibody: Human

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

Lot specific

Buffer

PBS,  pH 7.4, 0.09 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Avoid repeated freezing and thawing

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

CD19 (CD19 Molecule (CD19))

Andere Bezeichnung

CD19 (CD19 Produkte)

Hintergrund

Name/Gene ID: CD19

Synonyms: CD19, B-lymphocyte antigen CD19, CD19 Molecule, Differentiation antigen CD19, T-cell surface antigen Leu-12, B4, CD19 antigen, CVID3

Gen-ID

930

UniProt

P15391

Pathways

Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung